@@ -63,8 +63,14 @@ sct_deepseg -h
6363# Vertebral labeling
6464# ======================================================================================================================
6565
66- # Vertebral labeling
67- sct_label_vertebrae -i t2.nii.gz -s t2_seg.nii.gz -c t2 -qc ~ /qc_singleSubj
66+ # Vertebral disc labeling
67+ sct_deepseg totalspineseg -step1-only -i t2.nii.gz -qc ~ /qc_singleSubj
68+
69+ # Full spinal segmentation (Vertebrae, Intervertebral discs, Spinal cord and Spinal canal)
70+ # Segment using totalspineseg
71+ sct_deepseg totalspineseg -i t2.nii.gz -qc ~ /qc_singleSubj
72+ # Check results using FSLeyes
73+ fsleyes t2.nii.gz -cm greyscale t2_step1_canal.nii.gz -cm YlOrRd -a 70.0 t2_step1_cord.nii.gz -cm YlOrRd -a 70.0 t2_step1_levels.nii.gz -cm subcortical -a 70.0 t2_step1_output.nii.gz -cm subcortical -a 70.0 t2_step2_output.nii.gz -cm subcortical -a 70.0 &
6874# Check QC report: Go to your browser and do "refresh".
6975# Note: Here, two files are output: t2_seg_labeled, which represents the labeled segmentation (i.e., the value
7076# corresponds to the vertebral level), and t2_seg_labeled_discs, which only has a single point for each
@@ -80,11 +86,11 @@ sct_label_vertebrae -i t2.nii.gz -s t2_seg.nii.gz -c t2 -qc ~/qc_singleSubj
8086# ======================================================================================================================
8187
8288# Compute cross-sectional area (CSA) of spinal cord and average it across levels C3 and C4
83- sct_process_segmentation -i t2_seg.nii.gz -vert 3:4 -vertfile t2_seg_labeled .nii.gz -o csa_c3c4.csv
89+ sct_process_segmentation -i t2_seg.nii.gz -vert 3:4 -discfile t2_step1_levels .nii.gz -o csa_c3c4.csv
8490# Aggregate CSA value per level
85- sct_process_segmentation -i t2_seg.nii.gz -vert 3:4 -vertfile t2_seg_labeled .nii.gz -perlevel 1 -o csa_perlevel.csv
91+ sct_process_segmentation -i t2_seg.nii.gz -vert 3:4 -discfile t2_step1_levels .nii.gz -perlevel 1 -o csa_perlevel.csv
8692# Aggregate CSA value per slices
87- sct_process_segmentation -i t2_seg.nii.gz -z 30:35 -vertfile t2_seg_labeled .nii.gz -perslice 1 -o csa_perslice.csv
93+ sct_process_segmentation -i t2_seg.nii.gz -z 30:35 -discfile t2_step1_levels .nii.gz -perslice 1 -o csa_perslice.csv
8894
8995# A drawback of vertebral level-based CSA is that it doesn’t consider neck flexion and extension.
9096# To overcome this limitation, the CSA can instead be computed using the distance to a reference point.
@@ -96,7 +102,7 @@ sct_process_segmentation -i t2_seg.nii.gz -pmj t2_pmj.nii.gz -pmj-distance 64 -p
96102
97103# The above commands will output the metrics in the subject space (with the original image's slice numbers)
98104# However, you can get the corresponding slice number in the PAM50 space by using the flag `-normalize-PAM50 1`
99- sct_process_segmentation -i t2_seg.nii.gz -vertfile t2_seg_labeled .nii.gz -perslice 1 -normalize-PAM50 1 -o csa_PAM50.csv
105+ sct_process_segmentation -i t2_seg.nii.gz -discfile t2_step1_levels .nii.gz -perslice 1 -normalize-PAM50 1 -o csa_PAM50.csv
100106
101107
102108
@@ -127,7 +133,7 @@ sct_compute_compression -i t2_compressed_seg.nii.gz -vertfile t2_compressed_seg_
127133cd ../t2
128134
129135# Create labels at C3 and T2 mid-vertebral levels. These labels are needed for template registration.
130- sct_label_utils -i t2_seg_labeled .nii.gz -vert-body 3,9 -o t2_labels_vert.nii.gz
136+ sct_label_utils -i t2_step1_levels .nii.gz -keep 3,9 -o t2_labels_vert.nii.gz
131137# Generate a QC report to visualize the two selected labels on the anatomical image
132138sct_qc -i t2.nii.gz -s t2_labels_vert.nii.gz -p sct_label_utils -qc ~ /qc_singleSubj
133139
@@ -137,14 +143,14 @@ sct_qc -i t2.nii.gz -s t2_labels_vert.nii.gz -p sct_label_utils -qc ~/qc_singleS
137143# sct_label_utils -i t2.nii.gz -create-viewer 3,4,5 -o labels_disc.nii.gz -msg "Place labels at the posterior tip of each inter-vertebral disc. E.g. Label 3: C2/C3, Label 4: C3/C4, etc."
138144
139145# Register t2->template.
140- sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -l t2_labels_vert.nii.gz -c t2 -qc ~ /qc_singleSubj
146+ sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -ldisc t2_labels_vert.nii.gz -c t2 -qc ~ /qc_singleSubj
141147# Note: By default the PAM50 template is selected. You can also select your own template using flag -t.
142148
143149# Register t2->template with modified parameters (advanced usage of `-param`)
144- sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -l t2_labels_vert.nii.gz -qc ~ /qc_singleSubj -ofolder advanced_param -c t2 -param step=1,type=seg,algo=rigid:step=2,type=seg,metric=CC,algo=bsplinesyn,slicewise=1,iter=3:step=3,type=im,metric=CC,algo=syn,slicewise=1,iter=2
150+ sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -ldisc t2_labels_vert.nii.gz -qc ~ /qc_singleSubj -ofolder advanced_param -c t2 -param step=1,type=seg,algo=rigid:step=2,type=seg,metric=CC,algo=bsplinesyn,slicewise=1,iter=3:step=3,type=im,metric=CC,algo=syn,slicewise=1,iter=2
145151
146152# Register t2->template with large FOV (e.g. C2-L1) using `-ldisc` option
147- # sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -ldisc t2_seg_labeled_discs .nii.gz -c t2
153+ # sct_register_to_template -i t2.nii.gz -s t2_seg.nii.gz -ldisc t2_step1_levels .nii.gz -c t2
148154
149155# Register t2->template in compressed cord (example command)
150156# In case of highly compressed cord, the algo columnwise can be used, which allows for more deformation than bsplinesyn.
@@ -426,11 +432,6 @@ sct_deepseg sc_canal_t2 -i t2.nii.gz -qc ~/qc_singleSubj
426432# Check results using FSLeyes
427433fsleyes t2.nii.gz -cm greyscale t2_canal_seg_seg.nii.gz -cm red -a 70.0 &
428434
429- # Full spinal segmentation (Vertebrae, Intervertebral discs, Spinal cord and Spinal canal)
430- # Segment using totalspineseg
431- sct_deepseg totalspineseg -i t2.nii.gz -qc ~ /qc_singleSubj
432- # Check results using FSLeyes
433- fsleyes t2.nii.gz -cm greyscale t2_step1_canal.nii.gz -cm YlOrRd -a 70.0 t2_step1_cord.nii.gz -cm YlOrRd -a 70.0 t2_step1_levels.nii.gz -cm subcortical -a 70.0 t2_step1_output.nii.gz -cm subcortical -a 70.0 t2_step2_output.nii.gz -cm subcortical -a 70.0 &
434435
435436# Segment the spinal nerve rootlets
436437sct_deepseg rootlets -i t2.nii.gz -qc ~ /qc_singleSubj
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