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Copy file name to clipboardExpand all lines: learning-pathways/mags.md
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@@ -60,19 +60,19 @@ pathway:
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- name: quality-control
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topic: sequence-analysis
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# - section: "Module 2: Contamination and Host Reads Removal – Purifying Your Metagenomic Dataset"
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# description: |
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# Metagenomic datasets frequently contain **non-microbial sequences**, such as **host DNA** (e.g., from honey bees) or **external contaminants** (e.g., human DNA introduced during sample handling or sequencing). These sequences can distort downstream analyses, leading to **misassemblies, incorrect taxonomic assignments, and biased functional interpretations**.
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#
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# In this module, you will:
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# - Recognize the **sources and impacts** of contamination in metagenomic datasets.
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# - Learn how to **identify and filter out host and contaminant sequences** using Galaxy tools.
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# - Ensure your dataset is **enriched for microbial reads**, improving the accuracy of MAG reconstruction and enabling more reliable biological insights.
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#
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# By the end of this module, you'll be able to confidently clean your metagenomic data, setting the stage for high-quality MAG assembly and analysis.
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# tutorials:
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# - name:
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# topic: microbiome
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- section: "Module 2: Contamination and Host Reads Removal – Purifying Your Metagenomic Dataset"
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description: |
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Metagenomic datasets frequently contain **non-microbial sequences**, such as **host DNA** (e.g., from honey bees) or **external contaminants** (e.g., human DNA introduced during sample handling or sequencing). These sequences can distort downstream analyses, leading to **misassemblies, incorrect taxonomic assignments, and biased functional interpretations**.
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In this module, you will:
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- Recognize the **sources and impacts** of contamination in metagenomic datasets.
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- Learn how to **identify and filter out host and contaminant sequences** using Galaxy tools.
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- Ensure your dataset is **enriched for microbial reads**, improving the accuracy of MAG reconstruction and enabling more reliable biological insights.
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By the end of this module, you'll be able to confidently clean your metagenomic data, setting the stage for high-quality MAG assembly and analysis.
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tutorials:
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- name: host-removal
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topic: microbiome
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- section: "Module 3: Assembly – Reconstructing and Assessing Contigs from Metagenomic Reads"
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description: |
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topic: genome-annotation
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- name: amr-gene-detection
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topic: genome-annotation
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- section: "Recommended follow-up tutorial"
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description: |
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While this learning pathway goes into detail about all steps required for MAGs generation, a full end-to-end training was developed, which executes all workflows for MAGs generation and annotation instead of running individual steps. This tutorial can be used as a guide to execute the workflows on your own data. It explains how you can perform quality control, remove host contamination, and run the main MAGs workflow to obtain quality-controlled, taxonomically and functionally annotated MAGs.
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